| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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Purity: ≥98%
m-Cl-CCP [555-60-2] is a protonophore (h+ ionophore) and uncoupler of oxidative phosphorylation in mitochondria, inhibiting secretion of hepatic lipase and partially inhibiting the ph gradient-activated cl- uptake and cl-/cl- exchange activities in brush-border membrane vesicles
| ln Vitro |
CCCP prevents STING, TBK1, and IRF3 from being phosphorylated by interfering with their ability to bind to each other. STING translocation to the perinuclear region is not inhibited by CCCP, but it does interfere with the activation of STING and its downstream signaling molecules, TBK1 and IRF3. In addition to inducing mitochondrial fission, CCCP hinders the response between STING and TBK1. It's significant to note that STING activity was restored upon knockdown of the mitochondrial fission regulator Drp1, suggesting that CCCP inhibits the DMXAA-triggered STING signaling dye by causing the protonophore CCCP to lose its membrane potential. When RAW264.7 cells and MEFs are treated with DMXAA, CCCP dramatically reduces IFN-β production [1]. To detect mitosis, 1 μM CCCP is enough. There was minimal induction of mitosis in cells treated with 10 μM CCCP, the dose used to induce mitophagy. Due to mitochondrial avoidance binding to inward movement proteins, mitosis mechanistically requires the positioning of failed mitochondria at the cell periphery [4].
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| ln Vivo |
Apply CCCP and PPEF at the same dose of 3 mg/kg.bw each. There was a 1-log decrease in the bacterial burden in both situations. However, a 6 log10 decrease in bacterial counts was noted when 3 mg/kg.bw of PPEF and 3 mg/kg.bw of CCCP were combined. The combination treatment's increased antibacterial activity was confirmed by the created model [2]. Tc-MIBI signal in 99mSD scaffolds administered CCCP (4 mg/kg peritoneal) The 31P Seattle spectroscopic signal indicated a reduction in ATP concentration in scaffolds treated CCCP, as did the 99mTc-MIBI signal. We examined the isotope activity in the separated heart tissue of the scaffold that was supplied CCCP in order to determine whether CCCP decreased 99mTc-MIBI. The 99mTc-MIBI signal in the heart of the CCCP group was substantially lower than the heart rate 180 minutes after the injection of 99mTc-MIBI [3].
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| References |
[1]. Kwon D, et al. Carbonyl cyanide 3-chlorophenylhydrazone (CCCP) suppresses STING-mediated DNA sensing pathway through inducing mitochondrial fission. Biochem Biophys Res Commun. 2017 Aug 30. pii: S0006-291X(17)31704-7.
[2]. Sinha D, et al. Synergistic efficacy of Bisbenzimidazole and Carbonyl Cyanide 3-Chlorophenylhydrazonecombination against MDR bacterial strains. Sci Rep. 2017 Mar 17;7:44419. [3]. Kawamoto A, et al. Measurement of technetium-99m sestamibi signals in rats administered a mitochondrial uncoupler and in a rat model of heart failure. PLoS One. 2015 Jan 16;10(1):e0117091. [4]. Kondapalli C, et al. PINK1 is activated by mitochondrial membrane potential depolarization and stimulates Parkin E3 ligase activity by phosphorylating Serine 65. Open Biol. 2012 May;2(5):120080. [5]. Haifeng Jiao, et al. Mitocytosis, a migrasome-mediated mitochondrial quality-control process. Cell. 2021 May 27;184(11):2896-2910.e13 |
| Molecular Formula |
C9H5CLN4
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|---|---|
| Molecular Weight |
204.617
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| Exact Mass |
204.0203
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| CAS # |
555-60-2
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| Related CAS # |
555-60-2;
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| SMILES |
N#C/C(C#N)=N/NC1=CC=CC(Cl)=C1
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| InChi Key |
UGTJLJZQQFGTJD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C9H5ClN4/c10-7-2-1-3-8(4-7)13-14-9(5-11)6-12/h1-4,13H
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| Chemical Name |
Carbonyl cyanide 3-chlorophenylhydrazone
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| Synonyms |
CCCP Mesoxalonitrile 3-chlorophenylhydrazone
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~50 mg/mL (~244.36 mM)
H2O : < 0.1 mg/mL |
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (12.22 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.8871 mL | 24.4355 mL | 48.8711 mL | |
| 5 mM | 0.9774 mL | 4.8871 mL | 9.7742 mL | |
| 10 mM | 0.4887 mL | 2.4436 mL | 4.8871 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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